The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Novel Vacuum Manifold Eliminates Waste Handling Plate Design Prevents Cross-Contamination During Sample Processing For Both Manual and Automated Formats Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol: chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation. The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15â€"20" of Hg or equivalent and the Vac-Man 96 Vacuum Manifold or similar manifold. The Vac-Man 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).
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